The Characterization of Bacterial Metabolites by Atmospheric Solids Analysis Probe-Ion Mobility-Mass Spectrometry (ASAP-IM-MS) Methodologies

摘要

The diverse range of biological activities of metabolites has been attributed to the equally diverse and unique structural features of this class of molecules. IM-MS techniques have demonstrated considerable utility in life sciences research as it provides rapid 2D separations on the basis of collision cross section (Ω), which is related to ion structure, and m/z [1]. Atmospheric solids analysis probe (ASAP)-IM-MS analysis shows promise for rapid metabolomic profiling as molecules of interest are readily separated from chemical and biological noise in 2D IM-MS space. Secondary metabolites can be prioritized by the deviation from class-specific correlation lines in mobility space attributed to biomolecular classes (lipids>peptides>carbohydrates). With no prior sample preparation and sample analysis times on the order of seconds/sample, the robust methodology described here can be applied to various bacterial samples to study metabolites of interest.