Identification of Phosphorylation of Tyrosines on Targeted Proteins by Organophosphorus Pesticides using LC-MS/MS

摘要

CPO could covalently modify tyrosines as free amino acids as well as in intact proteins; CPO phosphorylation has selectivity based on peptide sequence (experiment 1) and protein structures (experiment 2); This protocol can be used for similar studies on other proteins, such as tubulin, kinesin and dynein, to investigate the effects of CPO on axonal transport.