Rapid Determination of Urinary Creatinine Levels Using Atmospheric Pressure Thermal Desorption Combined with Ion Mobility-Mass Spectrometry

摘要

The direct on-probe derivatisation of creatinine in urine allows the thermal desorption of acylcreatinine from the probe at low temperatures (<100°C). On-probe derivatisation followed by TD-EESI-IM-MS/MS provides a rapid procedure for the identification of creatinine in urine, and quantitation using a deuterated internal standard, with minimal sample preparation. The thermal desorption technique/extractive electrospray procedure demonstrated here could be applied to the direct analysis of other metabolites by using appropriate derivatisation agents to enhance volatility and target specific functional groups.