The GlycoFilter: A Comprehensive Sample Preparation Platform for Proteomics N-glycomics, and N-glycosylation Sites Assignment

摘要

N-glycans can be purified from proteins by simple filtration Deglycosylation in H_2 ~(18)O will minimize the false site assignments derived from the chemical deamidation Partial Occupancy in N-glycosylation seems common, even in healthy urine and plasma. Protein quantitation by sugar-based enrichment strategies may result in inaccurate quantitation of glycoproteins.