Coupling Free-Flow Electrophoresis with Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for Proteomic Analysis

摘要

Findings (1) Stream profile appears stable through open edge interface. (2) Proteins have been separated on an open edge μFFE device. (3) Proteins have been deposited on a protein capturing membrane. (4) DESI-MS of myoglobin and BSA off of glass and myoglobin off of a membrane have been successfully performed. (5) Proteins were successfully transferred from open edge μFFE device to membrane by use of a SNAP i.d. 2.0 device (Merck, Darmstadt, Germany) and house vacuum. Next steps Next steps (1) Further optimization of membrane/device interface with voltage applied. (2) Desorption of protein off of membrane after separation. (3) Viability of on membrane protein concentration and wash