LC-LC-MS/MS Heart-cut Approach for Software assisted Pharmaceutical Impurity Identification from non-ms compatible LC method Using Time of Flight Mass spectrometer

摘要

This workflow demonstrates a Heart cut two dimensional LC/MS method for selective identification of an impurity. This approach eliminated need to develop an equivalent MS compatible LC method, for a non-MS compatible pharmacopeia method to perform accurate mass analysis of the impurity of the interest. The Heart cut two dimensional LC/MS method enabled specific selection of a close eluting, low abundant unknown impurity in the API for MS analysis. Agilent's 6540 Q-TOF along with advanced software tools (MSC, MFE, and MFG) enables quick and reliable identification of unknown compounds.