Proteomic analysis of pre-sound wave stimulation on the change of protein in Arabidopsis infected by Botrytis cinerea using MALDI TOF/TOF MS

摘要

Plants possess highly complex sensory mechanisms to perceive dynamic surroundings and to adapt to predominant environmental conditions. The mechanisms by which sound influences plant growth, however, remain obscure. Previously, our group reported that several proteins were differentially regulated by specific frequencies of sound treatment using a sound-treated subtractive library. In this study, we used a proteomic approach to the influence of pre-sound wave stimulation on the change of proteins in Arabidopsis infected by Botrytis cinerea. The plants were infected by Botrytis cinerea after treatement with 1000-Hz sound wave and total proteins were extracted from leaves 12, 24, 48, 72, 96, and 120 h after treatment. Seventy proteins were found to be differentially regulated by sound waves and were identified MALDI-TOF/TOF MS. The functions of the identified proteins were classified into stress and defense, amino acid metabolism, carbohyderate metabolism, photosynthesis, antioxidant enzyme, energy metabolism, nucleotide metabolism, nitrogen metabolism and other metabolism. To the best of our knowledge, this is the first report on the analysis of protein changes in response to specific sound-waves in Arabidopsis infected by phytophthogenic fungi. These findings provide a better understanding of the molecular basis of responses to sound waves in Arabidopsis.