Biomarker Discovery for Radiation Induced Tissue Damage Via Targeted and Un-Targeted Mass Spectrometry Based Metabolomics

摘要

Integrated mass spectrometric approach incorporating targeted and un-targeted metabolomics for biomarker discovery and validation from plasma and tissue fromwell-defined radiation exposure animal models. Established connection between plasma citrulline concentration and histopathological alterations in the small intestines following radiation exposure. (1) Crypt survival and citrulline concentration were dose dependent; (2) Citrulline levels appear to reflect enterocyte mass. Retinoic acid concentration is reduced in both lung and GI tissue after radiation insult: (1) The degree of depletion of RA and retinol was evident across the GI tract (jejunum and colon); (2) Demonstrated RA concentration in lung tissue was significantly reduced upon high dose irradiation across three phenotypically diverse mice strains. Actively pursuing un-targeted metabolomic profiling for identifying potential radiation-linked biomarkers.