Hexavalent Chromium-Induced Alteration of Proteomic Landscape in Human Skin Fibroblast Cells

摘要

By using SILAC combined with LC-MS/MS analysis, we were able to achieve a quantitative assessment of the K_2Cr_2O_7-induced perturbation of the proteome of GM00637 cells. Particularly, we demonstrated, for the first time, that Cr(VI) may exert its cytotoxic effect, at least in part, through elevating endogenous cholesterol biosynthesis. The novel insight into the cytotoxicity of Cr(VI) provides the basis for the future development of effective approaches for the therapeutic intervention following Cr(VI) exposure.