METHOD FOR PRODUCING A MIXTURE FOR SPRAYING HUMAN KERATINOCYTES CELLS-FIBROBLASTS IN SOLUTION AND ITS APPLICATION AS A REGENERATIVE AGENT FOR SKIN DAMAGE

摘要

The present invention relates to a process for obtaining a compound of cellular spraying for fibroblasts and human keratinocytes and its use as regenerative agent of cutaneous lesions, mainly in diabetic ulcers, first, second, and third grade burns, and a substitute for the use of flaps techniques. The process for obtaining a compound of cellular spraying for fibroblasts and human keratinocytes includes the steps of separating the dermis and epidermis layers, incubation of the epidermis layer, disintegration of dermis layer and proliferation of fibroblasts, disintegration of the epidermis layer, proliferation of keratinocytes in suspension, preparation of the cross-linking reaction agent solution of the cellular spraying solution, and preparation of cellular spraying solution. The compound for cellular spraying of fibroblasts and human keratinocytes increases the application efficiency and ease of it, in order to be an economic alternative, fast, and easy to use.