Objective: To investigate damage effects of ultraviolet irradiation on eternal keratinocyte-HaCaT cells and to evaluate photo-protective efficiency of hydroxychloroqine and Traditional Chinese Medicines ( epigallocatechingallate[EGCG], baikal skullcap root and szechwan lovge rhizome) on HaCaT ceils damaged by middle wave ultraviolet(UVB) irradiation. Methods: Subconfluent HaCaT cells were sham or UVB irradiated and treated with above TCM agents. The damage degree of HaCaT cells was observed by a light microscop. Cell growth was recorded by cellcount and cellular activity was detected by MTT method. The secretion amount of IL-6 and TNF-α was measured by ELISA. Results: The irradiation damage of HaCaT cells was depended on the irradiated dosages and cellular activity was reduced by 36%-80%, with a maximum decrease over 90% after 72 h. The intervention of the above drugs may increase the cellular activity by 10%-72%. The photo-protective efficiency was more apparent in F_A^G (from 1.19+0.07 to 1.28 + 0.06, P < 0.01 ) than that in hydroxychloroqine ( from 0.43 + 0.04 to 0.96 + 0.04, P < 0.05). The other two tested drugs also showed photo-protective effect( from 0.44 + 0.07 to 1.21 -+ 0.02, P < 0.05). As to cytokine secretion, EGCG could decline the secretion amount of IL-6 and TNF.a apparently. Hydroxychloroqine and baikal skullcap root baikal skullcap root could only reduce the secretion of ID6. The secretion of IL-6 and TNF-α could not be inhibitedby szechwan lovge rhizome. Conclusion : The injury effect of UVB irradiation on cultured keratinocytes is dose-dependent and the tested drugs have photo-protective potency. Inhibition of cytokine secretion may be one of the mechanisms related to reducing the damage effect of UVB irradiation.
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