Practical comparison of LC-MS/MS-based workflows for quantitation of desmosine and isodesmosine in urine - potential lung damage biomarkers in premature infants

摘要

Each of the tested methods can be applied to the quantitative analysis of DES and IDES in urine samples - however, each has advantages and disadvantages which must be considered in context of project requirements: (1) Methods B and C achieve baseline resolution of two isomeric analytes. However, current clinical data do not support the necessity of quantifying DES and IDES individually - so chromatographic separation, while satisfying analytically, may be extraneous; (2) Method A is the most MS compatible and would be best suite for large scale measurement of combined DES and IDES, as run time can be significantly shortened to provide high-throughput analysis; (3) Both methods B and C utilize ion paring agents; of the two, TF is more MS friendly than HFBA, in that it is more volatile, eas to wash out, and causes less ionization suppression; (4) Successful application of Method B requires extensive, time consuming sample preparation, while methods A and C work well with straight urine; (5) Due to its sensitivity, chromatographic resolution and lack of matrix interferences, Method C is our choice for this ongoing project.