Dynamics of acylsugar biosynthesis and metabolism in Solanum glanduar trichome development

摘要

Platforms for whole plant ~(13)C-CO_2 labeling plus LC-MS profiling were set up for investigation of dynamics of glandular trichome metabolism. The unequal rates of label incorporation and disappearance in metabolites and substructure fragments provide temporal information about rates of synthesis, degradation, and transport of the Solanum trichome metabolome. Constant levels of primary metabolites and turnover of acylsugar in tomato plants indicates a potential pathway that leads to degradation of the molecule after early stage of development (2 weeks post-germination), suggestive of a genetic basis (e.g. hydrolases) that limits acylsugar accumulation.