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TRICHOME-SPECIFIC TRANSCRIPTION FACTOR MODULATING TERPENE BIOSYNTHESIS

机译:TRICHOME特定转录因子调节萜的生物合成

摘要

The present invention relates to identification and isolation of zinc finger transcription factor in tomato that specifically expresses in glandular trichomes of Solanum lycopersicum cultivar Moneymaker and binds to the promoters of the genes encoding Terpene Synthase 5 (also known as Monoterpene Synthase1) and Terpene Synthase 11 (also known as Sesquiterpene Synthase 1). The invention provides the isolated, recombinant or synthetic polynucleotides encoding the polypeptide sequences of SEQ ID NO:2 and variants and fragments thereof. The invention also provides constructs, vectors, host cells and plants genetically modified to contain the polynucleotides of the invention. The methods for producing plants with altered levels of terpenes, including transformed and mutant plants, are also provided.
机译:本发明涉及番茄中锌指转录因子的鉴定和分离,该锌指转录因子特异性地在 Solanum lycopersicum 品种Moneymaker的腺毛状体中表达,并与编码萜烯合酶5(也称为单萜)的基因的启动子结合。合酶1)和萜烯合酶11(也称为倍半萜烯合酶1)。本发明提供了编码SEQ ID NO:2的多肽序列及其变体和片段的分离的,重组的或合成的多核苷酸。本发明还提供了经基因修饰以包含本发明的多核苷酸的构建体,载体,宿主细胞和植物。还提供了生产具有改变的萜烯水平的植物的方法,包括转化的和突变的植物。

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