Super-SILAC based quantitative proteomics for comparison of different acute myeloid leukemia cell lines

摘要

Anumber of significant protein expression changes were found between the cell lines, including proteins related to cancer pathways which can complement previous genetic and cytogenetic studies. Only 40 % of the proteins were quantified in all five cell lines. By combining five cell lines as internal standard we cover the AML proteome to a larger extend than by using only one, which is an advantage if using super-SILAC mix for comparison of patient samples.