Quality Control Measures for Routine, High-Throughput Targeted Protein Quantitation Using Tandem Capillary Column Separation

摘要

Incorporation of the PRTC kit increases the capabilities of tandem LC experiments for targeted protein quantitation: (1) Increases throughput while maintaining low flow rates and longer gradients necessary to maintain peak capacities; (2) Provides direct retention-time prediction using all types of LC methods on all C18-based LC columns; (3) Facilitates implementation of different LC experiments using tandem LC to maximize sensitivity for different sets of targeted peptides; (4) Facilitates internal RT and QC landmarks for each experiment for normalization based on the breadth of the hydrophobicity factors for the PRTC peptides.