A PROCESS FOR SEPARATION AND QUANTITATION OF PROTEINS USING CAPILLARY ELECTROPHORESIS

摘要

The present disclosure provides a method for analyzing, detecting and separating at least one low molecular weight impurity from a protein mixture using capillary electrophoresis, e.g., capillary electrophoresis - sodium dodecyl sulfate (CE-SDS). The present disclosure further provides methods to improve protein peak separation efficiency and quantification of a protein. Furthermore, the present disclosure provides an improved reduced CE-SDS method for analyzing a protein mixture comprising protein of interest which is pegylated and separates LMW or HMW fragments present in the protein mixture.