Enhanced selectivity in the qualitative and quantitative analysis of peptides using in-source fragmentation of FAIMS-selected ions combined with LC-MS

摘要

1. FAIMS has been combined with LC-MS for the selective transmission of differential mobility pre-selected ions prior to in-source CID (LC-FISCID-MS). 2. Transmission of FAIMS-selected peptide ions enhances the detection of characteristic fragment ions of the selected peptides, generated by in-source CID, by eliminating fragments associated with co-eluting peptides 3. The quantitative potential LC-FISCID-MS has been demonstrated for the determination of an exogenous peptide spiked into human plasma.