Quantitative 3D Proteomics Reveals Protein Conformation Changes Using Isotope-labelled Cross-linkers

摘要

3D proteomics, a combination of chemical cross-linking, mass spectrometry and database searching, has been increasingly successful in providing medium resolution data on static protein structures. This has been shown by our lab analysing 0.5 MDa and 0.7 MDa Pol II complexes and also by other labs. Integrating quantitation with 3D proteomics promises to provide insights into the structural dynamics of proteins. Here we demonstrate the possibility of using isotope-labelled cross-linkers for quantitation, a strategy that focuses quantitation on cross-linker containing species and places minimal restriction on the protein source.