Higher Resolution Improves Top-Down Protein Identification Results on an Orbitrap Mass Spectrometer for Large (>40 kDa) Proteins

摘要

1. Higher resolution is highly beneficial for improving confidence and sequence coverage for identification of larger proteins. 2. High resolution is necessary to iden tify large proteins using LC-MS/MS. 3. The 480,000 resolution mode shows promise for further improvements in the confidence of top-down pr otein identifications. 4. ProSightPC software version 3.0 is well suited for identification of complex intact protein mixtures.