Development and Application of a Quantitative Proteomics Approach for Identifying Protein-Drug Interactions in Complex Biological Mixtures

摘要

1. Targeting peptides in LC-MS/MS and purifying proteins is a useful technique to determine validity of potential target. 2. Purified protein analysis can validate direct interactions. 3. Type II HSP40 co-chaperone may be a direct protein target of 4-OH Tamoxifen and Tamoxifen. 4. Further validation of Type II HSP40 and the other 16 proteins is still needed. 5. 2 Protein Hits, Fatty acid synthetase and 6-phosphoglucose isomerase, need additional validation, but there is a previous established connection with Tamoxifen.