Targeted Cysteine Peptide Analysis in Complex Protein Samples using 351 nm UVPD Thiol-Selective Tags

摘要

1. Site-selective modification of cysteine residues with Alexa Fluor 350 maleimide significantly increases gas phase absorptivity near 350 nm. 2. Selective dissociation of cysteine-containing peptides was achieved using 351 nm UVPD in conjunction with Alexa Fluor 350 derivatization. 3. The proposed targeted proteomic workflow yielded sequence information exclusively from modified cysteine-containing peptides in complex protein matrices, resulting in significantly streamlined datasets.