Quantitative Analysis of 18-beta-Glycyrrhetinic Acid, Cortisol and Cortisone by LC-MS/MS for Determination of 11 beta-HSD2 Enzyme Activity in Patients

摘要

The DoE was useful for evaluation of the factors and optimization of the method. The UPLC-MS/MS assay method provides significant improvements in sensitivity and analysis run time for simultaneous quantification of the analytes in both plasma and urine from humans. We have successful developed a validated method for quantitative determination of 18-beta-Glycyrrhetinic Acid in human plasma over the range of 3.0 - 2000 ng/ml. This method was implemented for analyses of samples from individuals with and without liquorice consumption.