MS/MS fragmentation patterns and efficiency depends on the dissociation methods (CID and ETD), the sequence of the tetrasachharides, and the type of metal used. Doubly charged metal-adducted tetrasaccharides undergo ETD and CID by both cross-ring and glycosidic cleavages. Complementary structural information for metal-adducted oligosaccharides can be obtained by comparing ETD spectra with CID spectra. The extent of fragmentation of doubly charged metal-adducted tetrasaccharides by ETD follow the trends: Transition Metals > Alkaline Earth Metals > Alkali Metals Maltotetraose > Stachyose > Galactotetraose > Nystose.
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