Integrated Top-down and Bottom-up Proteomics of Natural Abundant and Heavy Isotope Depleted Saccharomyces Cerevisiae using LC-FT-ICR and LC-Ion Trap MS

摘要

Over 1,000 yeast biomolecules are observed, presumably proteins, ranging from 1 to 35 kDa, which extends the number obtained from yeast cell lysates using top-down method from the previous record of 231 protein species detected. 1018 proteins are identified using 2D-LC ion trap MS with bottom-up method from yeast cell lysates. 35 proteins (91 peptides) from top-down data were identified by correlation with proteins identified with bottom-up method.