A Mass Spectrometric Assay for Analysis of Haptoglobin Fucosylation in Pancreatic Cancer

摘要

A highly-sensitive analytical strategy is developed to elucidate N-glycan structures and evaluate fucoyslation for target proteins in human cancer serum. A tri-antennary complex type glycan with both core and antennary fucosylation was identified for the first time in haptoglobin in pancreatic cancer. This glycan as well as another bifucosylated tetra-antennary glycan are unique in pancreatic cancer. Fucosylation degree index was utilized to provide a numerical depiction of haptoglobin fucosylation differences between pancreatic cancer and other benign conditions. Elevation of haptoglobin fucosylation is present in all stages of pancreatic cancer including the early stage, revealing the potential of early detection.