Metabolomics: Fast UPLC/MS/MS targeted MRM analysis of L-arginine, homoarginine, ADMA and SDMA levels in serum and tissue samples

摘要

Monitoring the levels of L-arginine and its analogs asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA), homoarginine and citrullin in biological fluid and tissue is important since elevated levels of these molecules have been associated with many human diseases includes chronic heart failure, hypercholesterolemia, atherosclerosis, hypertension, chronic renal failure, and other clinical conditions such as COPD. Asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of nitric oxide (NO) synthase. ADMA inhibits vascular NO production at concentrations found in pathophysiological conditions: (1) Little is known about arginine metabolism in the pathogenesis of chronic inflammatory airway diseases including asthma and chronic obstructive pulmonary disease (COPD). (2) Several studies have investigated the relationship between cigarette smoke and ADMA levels, with conflicting results. (3) There is no direct ADMA measurements in COPD or asthma patients.