Proteins and genes induced by hepatotoxicity of Cisplatin, an anticancer drug

摘要

We identified the proteomics and genomics profiles of rat primary hepatocytes treated with cisplatin using nano LC-MS/MS and microarray, respectively. Many liver proteins and pathways modulated in the response to cisplatin treatment have been identified in the present study. Among many pathways, we focused on some pathways including drug metabolism, fatty acid metabolism, glycolysis and TCA cycle, urea cycle, and inflammation metabolism pathways. Pathway and network analyses provided an integrated picture of the hepatotoxic effects of cisplatin in rat hepatocytes. By comparing proteomic profiles with genomic profiles, the present study provides information useful for an understanding of the roles of these alterations in regard to the cytotoxicity and toxic mechanisms of cisplatin. Finally, this study represents the first application of both proteomic and genomic analysis on cisplatin-treated rat primary hepatocytes and provides novel insight into the potential toxic pathways induced by cisplatin.