ESI-MS Investigation of the Binding of Human Hemoglobin to the N-terminal Domain of Erythrocyte Band 3

摘要

Hb binds band 3 full length peptide in 1:1 ratio. Aqua-metHb binds full length peptide with the highest affinity, while COHb with the lowest. CN-metHb binds full length peptide with similar affinity to Aqua-metHb. CN-metHbS binds acetylated band 3 peptide (#2) with higher affinity than CN-metHbA, while both the CN-met HbA and HbS bind the nonacetylated band 3 N-terminal first 14 residues (peptide #1) with about equal strength.