Quantitative Investigation of Peptide Biomarker Stability in Blood Specimens by comparing MS and ELISA Methods

摘要

Human blood specimens utilized extensively as attractive samples contain a variety of disease information, and blood-derived plasma and serum are readily available for proteomic and/or clinical analysis. Recent plasma proteomic research has led to the discovery of a growing number of potential biomarkers required further validation. Yet, among a variety of the recently identified barriers that inhibit the translation of the discovered biomarker into clinical application, proteolytic activity intrinsic to the blood causes instability and variability of protein and peptide biomarkers. Our previous work has demonstrated that biomarker half-lives can be extended in the presence of protease inhibitors. Here we further investigate the peptide biomarker instability at different concentration in whole blood and plasma samples through comparing MS and ELISA methods.