Glycosylation Profiling of Therapeutically Active Glycoproteins: Identification of Glycans in Recombinant Factor IX using Directed MALDI-QIT-TOF-MS~(n) and a Glycan Database

摘要

We have tested our Accurate Glycan Analyser workflow by easily detecting and identifying glycans from known glycoproteins, such as fetuin. We have shown one application aimed at the quality control of therapeutically active glycoproteins (blood coagulation Factor IX). It is a challenge nowadays to produce recombinant, therapeutically active glycoproteins. Our example demonstrate this fact, as significant differences were observed between plasma-derived- and recombinant-FIX: the extent of fucosylation and the core structures of the identified glycans were the main inconsistencies between these two endogenous glycoproteins.