UPLC-MS/MS Analysis of Bimatoprost and its Free Acid Metabolite from Minipig Skin

摘要

The best homogenization was obtained using heat and high-pH to incubate the pigskin prior to homogenization on the FastPrep~(~R) bead-beating homogenization apparatus. A salt-assisted liquid-liquid extraction was used to selectively extract the parent and the free acid from the non-polar matrix materials with 99percent overall recovery of bimatoprost and 84percent recovery of the free acid metabolite. Less than 0.5percent degradation of the parent was observed under the incubation and extraction conditions utilized. Reverse-phase UPLC proved to have similar sensitivity to the normal-phase chromatography previously utilized, but fit better within the workflow of a high-throughput bioanalytical laboratory. Standard and QC performance show this method is accurate and precise.