A Systems Biology Approach; Proteomic Study on Rapamycin Treated Human Cancer Cell Line

摘要

Glycolysis/gluconeogenesis pathway is reported to be affected by rapamycin treatment. In this study, down regulation of pyruvate kinase, glucose phosphate isomerase and up-regulation of malate dehydrogenase are observed, indicating glycolysis is reduced and gluconeogenesis is enhanced due to rapamycin. Up-regulation of the gene for glucosamine-6-phosphate deaminase 2 and elevation of glucose in metabolite analysis supports this observation. Up-regulation of Heat shock proteins, which is known to be a stress response is observed in both proteomics and gene expression analysis. Rapamycin treatment is reported to result in decreased protein levels. Observation of down regulation of eukaryotic translation initiation factor 4A in proteomic analysis and up-regulation of amino acids in the metabolite analysis indicates down regulation in protein synthesis. Proteomic, genomic and metabolomic analysis using various platforms enables better understanding of biological process.