Two pH Optimized LC-MS Methods for Metabolomics Analysis of Hydrophilic Compounds on a Silica Hydride Stationary Phase

摘要

Two optimized LC/MS methods for comprehensive coverage of hydrophilic metabolites were developed to build an Accurate Mass and Retention Time database. By switching from glass solvent bottles to HDPE trace sodium present in the solvents (leaching from the glass) was eliminated, which resulted in dramatic improvement of nucleotide (mainly ATP, GTP, ITP etc.) peak shape. The methods developed are rugged and applicable to physiological samples such as urine.