A Stress Response Comparison: LC/MS metabolomics analysis of Saccharomyces cerevisiae exposed to the immunosuppressant drugs FK506 and Cyclosporin A

摘要

Novel yeast metabolite extraction protocol yielded a high number of features detected using MFE. Using MPP, many compounds showed reproducible variation between immunosuppressant and calcium stress states. ANP chromatography was effective in separating many compounds co-eluted near the void volume with RP chromatography. The Agilent 6530 Accurate-Mass Q-TOF mass spectrometer operated in ESI positive mode with dual chromatographic applications enabled a robust non-targeted characterization of the S.cerevisiae metabolome. The Agilent Accurate Mass Retention Time database enables orthogonal confirmation of metabolites. Ms/ms analysis will be a useful tool for identifying unknown compounds of interest and making the challenging transition from non-targeted to targeted analysis.