Direct Measurement of Tetrahydrobiopterin, Dihydrobiopterin and Biopterin in Human Plasma and Breast Milk by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

摘要

Despite the extreme liability of some of the analytes a high throughput LC-MS/MS method has been developed for direct measurement of BH4, BH2 and B in human plasma. The LLOQ of this method was 10.0 nM (2.42 ng/mL) for BH4, 10.0 nM (2.40 ng/mL) for BH2, and 100 nM (23.8 ng/mL) for B. The performance of the method met the acceptance criteria which were recommended in the Guidance for Industry Bioanalytical Method Validation (Federal Drug Administration, CDER, CVM, May 2001) and white papers (Shah, V.P. et al, 1991; Shah, V.P. et al, 2000; Viswanathan, C.T. et al, 2007). With slight modification in sample extraction this method can also be used to measure BH4 in human breast milk.