Tandem Mass Tag (TMT) labeling developed for Peptidomics of Cerebrospinal Fluid: Investigations on pre-analytical sample handling influencing the CSF Peptidome

摘要

The analysis of native peptides in CSF is challenging due to its high complexity and sensitivity towards artificial proteolytic cleavage of abundant proteins. Therefore, we have developed a novel peptidomics workflow enhancing the quantitative measurement of peptides by using TMT labeling. Since the initial steps are labeling and mixing of clinical samples the native peptide pattern is conserved throughout the whole workflow.