Method Development for the Comprehensive Compositional Analysis of Heparin/Heparan Sulfate Disaccharides from Human Serum

摘要

An in-depth compositional analysis of heparin/HS-derived disaccharides from human serum is currently under way. Heparin/HS disaccharides were extracted effectively from human serum using the current protocol and detected by LC-MS. The method has a high recovery (70percent-110percent), good linearity (R~(2)>0.994) and a low detection limit (3.1 pmol-17.8 pmol) for the analysis of the heparin/HS disaccharide standards extracted from the LMW serum fraction. Using this method, variations in the heparin/HS disaccharide patterns in different human sera were observed.