Time-Resolved Proteomic and Genomic Studies Reveal that Replication Fork Progression is Remarkably Uniform Throughout the Yeast Genome

摘要

In mitotic division, cells duplicate their DNA in S-phase to ensure that the proper genetic material is passed on to their progeny. This process of DNA replication is initiated from several hundred specific sites, termed origins of replication, spaced across the genome. Bi-directional replication fork movement occurs subsequent to the firing of origins upon recruitment of the replicative helicase and the other fork-associated proteins as the cell enters S-phase. The replication machinery itself (polymerases, PCNA, etc.) trails behind the helicase, copying the newly unwound DNA in the wake of the replication fork. One component of the pre-RC, the GINS complex, consists of a highly conserved set of paralogous proteins (Psf1, Psf2, Psf3 and Sld5), and likely moves with the replication fork.