It is known that patients who are hypersensitive to peanuts tend to be more allergic to roasted peanuts. In our previous study, the primary structure, including post-translational modifications, of the four isoforms of Ara h 2 has been characterized using LC-MS/MS methods (1). This work also identified the location and arrangement of four intramolecular disulfide bonds. In the present study, we have applied immunological (2) and mass spectrometric methods to characterize differences between Ara h 2 from raw and dark roasted peanuts.
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机译：众所周知，对花生过度敏感的患者往往对烤花生更过敏。在先前的研究中，使用LC-MS / MS方法（1）表征了ARA H 2的四种同种型的主要结构，包括翻译后修饰。该工作还确定了四种分子内二硫键的位置和布置。在本研究中，我们施用了免疫学（2）和质谱法，以表征来自原料和深色烤花生的ARA H 2之间的差异。
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