One-Dimensional Capillary Liquid Chromatographic Separation Coupled with Tandem Mass Spectrometry Unveils Entire Proteome Expressed in Escherichia coli Yeast cells

摘要

The Long monolith silica column with long gradient time showed great performance on shotgun E. coli proteome analysis. In total, 22,196 non-redundant tryptic peptides from 2,602 proteins including 830 membrance proteins were identified for the #. coli cells (triplicate analyses), in which the equivalent number of genes was detected by DNA microarray analysis.