Optimal and robust analysis of high-mass-accuracy Orbitrap datasets using MaxQuant and ProteinPilot search algorithms.

摘要

MaxQuant Quant module generates an improved spectral processing and higher mass accuracy of precursor peaks. Dataset features such as amino acid frequency, digestion characteristics, mass and m/z distribution, Unused score distribution are similar as expected. Search with MaxQuant processed peak list, leads to better spectral utilization (more proteins, peptides and spectra).