Nanostructured Surfaces Produced by Laser Ablation as Substrates for Metabolome Analysis via Laser Desorption/Ionization Mass Spectrometry

摘要

Porous Silicon surfaces produced via anodic etching in HF have been used to compare different surface treatments to detect endogenous cellular metabolites. The loading on the nanostructured silicon of 3-aminopropyldimethylethoxysilane as initiator showed the best sensitivity for the analytes studied (according to the NIMS protocol). Detection of endogenous metabolites like ATP and Acetyl CoA was shown in a yeast cell lysate. An innovative method based on laser ablation by short IR laser pulses (150fs) was employed to produce nanostructured surfaces on a wide range of materials. The surfaces obtained have been characterized by SEM. The LDI capabilities of the substrates produced by femtosecond laser ablation have been evaluated by measuring different standards either in positive and negative ion mode. Silicon surfaces showed the best ion yield performance. Loading of initiator was also accomplished to increase the sensitivity of the substrates obtained.