Diamond Nanoparticles for MALDI-TOF Mass Analysis of Bacterial Surface and Secretion Proteins

摘要

Acinetobacter baumannii is a gram-negative pathogen and it causes an important percentage of nosocomial infections mainly affecting immunocompromised patients. This microorganism is almost invariably multi-drug resistant (MDR). Currently, one of the popular methods of generating protein profiles is sodium dodecyl sulfate-polyacrylamide gel electrophoriese (SDS-PAGE). However, it has the disadvantages concerning automation and field applications. Our previous study showed the putative sensor kinase bfmS genes were correlated with the bacterial surface or secretion proteins of A. baumannii. In this study, we present a novel and rapid microanalytical protein-based approach, diamond nanoparticles, as a high-affinity probe to capture bacterial surface or secretion protein for the analysis of difference of proteins expression between wild type strain and bfmS knockout mutant by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS). We found diamond nanoparticles captured more proteins with better resolution while SDS-PAGE analysis showed only fewer bands of proteins.