In-gel Digestion of Mouse Membrane Protein Extract: Increased Peptide Recovery and Identification of Very Low Abundance Hydrophobic Proteins

摘要

The use of a commercially available MS friendly surfactant decreases digestion time from overnight (16 hrs) to 1 hr Peptide identification is significantly enhanced when using the surfactant: (1) Very low abundance membrane proteins that otherwise went undetected in a routine in-gel digest were identified; (2) Sequence coverage was increased in over 50percent of the proteins identified. The most significant change in physiochemical properties found in the subset of peptides was a trend toward low GRAVY values (more hydrophilic peptides). Peptides identified with surfactant had a disproportional amount of alanine and aspartic acid.