Methodology for the Rapid Determination of Lipids Employing Chip-Based nano-ESI FT-ICR MS, Accurate Mass, and Infrared Heating

摘要

Lipids are a diverse class of biomolecules that are generally characterized by their hydrophobicity. Lipids perform many biological roles including contributions to structure, provision of energy, and molecular signaling. Mass spectrometric determination of lipid classes in biological samples normally relies on characteristic fragmentation patterns during MS/MS or retention time matching for LC-MS. Both of these methods require time for instrument optimization, data collection, and data analysis. Accurate mass measurements of lipids using FT-ICR MS and conversion of these accurate masses to the Kendrick mass scale allow for the rapid identification of lipid classes in biological samples. At roughly 95percent glyercides, bovine milk provides a complex yet controlled source of lipids with which to demonstrate the power of accurate mass measurements.