Performance Evaluation of Three LC/MS Methods Implemented on an Ion Trap Mass Spectrometer for Drug Testing in Urine

摘要

All 245 compounds screened with MS~(2)/MS~(3) and MS~(3) methods in spiked urine samples were identified. The probability of non-specific endogenous peak ion selection for spectra acquisition increases as the number of targeted compounds in screening method increases. Identification limits in methods with longer scan events cycle (MS~(2)/MS~(3) and MS~(3) methods) are affected more by collecting spectra for endogenous compounds than methods with shorter scan events cycle (MS~(2) method). Methods collecting MS~(2) and MS~(3) or just MS~(3) spectra provide stronger hit confirmation. They are less affected by interference but may result in higher detection limits for some compounds compared to method using just MS~(2) spectra.