Biocompatibility and Osteo-Differentiation Study of Poly (?-Caprolactone) and ?-Tricaicium Phosphate Composite Membranes

摘要

The Tissue Engineering appears with a modern proposal for the treatment of damages or diseases. The study of materials and methods for tissues and organs regeneration by the patient cells culture had been developed on the last years but still couldn't be used for all different tissues. In this multidisciplinary research field, the present work joins the biodegradability of poly(?-caprolactone) (PCL) with the osteoconductive properties of ?-tricalcium phosphate (?-TCP) in order to create a composite which acts as a temporary support for cell culture without a second surgery to remove the biomaterial. This work evaluates three membranes types, obtained by casting in chloroform, on the biocompatibility and differentiation on mesenquimal stem cells (hMSC). These analyses showed cell viability with the rezasurin method and the alkaline phosphatase activity (ALP). DMA analyses, MEV and OPM were performed.