目的：制备聚己内酯／Ⅰ型胶原蛋白／纳米羟基磷灰石复合材料用于可吸收性引导组织再生膜，并进行理化性能表征和生物相容性检测。方法采用静电纺丝技术制备聚己内酯／Ⅰ型胶原蛋白、聚己内酯／Ⅰ型胶原蛋白／纳米羟基磷灰石复合材料，通过扫描电子显微镜表征纤维形貌。牙周膜细胞接种于支架，扫描电镜观察细胞形貌，CCK －8法检测细胞增殖，实时荧光定量 PCR 检测牙周膜细胞的骨化分化情况。结果聚己内酯／Ⅰ型胶原蛋白、聚己内酯／Ⅰ型胶原蛋白／纳米羟基磷灰石呈复层多孔网状结构，聚己内酯／Ⅰ型胶原蛋白／纳米羟基磷灰石由于纤维内部包裹纳米颗粒致表面呈粗糙外观。扫描电子显微镜、CCK －8法检测技术显示牙周膜细胞在支架上伸展充分，稳定增殖；实时荧光定量 PCR 结果表明聚己内酯／Ⅰ型胶原蛋白／纳米羟基磷灰石复合材料表面牙周膜细胞中 Runt 相关转录因子2、骨钙蛋白的基因表达量均明显高于对照组，提示该材料能够诱导牙周膜细胞向成骨细胞方向分化。结论聚己内酯／Ⅰ型胶原蛋白／纳米羟基磷灰石复合材料无明显细胞毒性且具一定的骨化诱导潜能，有望作为一种新型可吸收性引导组织再生膜材料。%Objective To prepare the electrospun poly (ε-caprolactone)/type-I collagen anophase hydroxyapatite nanofiber,and to examine its characteristics and biocompatibility.The composite material is expected to be used as the biodegradable membrane for guided tissue regeneration.Methods The PCL /COLⅠ and PCL /COLⅠ HAP scaffolds were prepared by electrospinning technique and observed by scanning electronic microscope.The morphology,proliferation and differentiation of periodontal ligament cells seeded on the scaffolds were evaluated by scanning electronic microscope,CCK-8 assay and real-time quantitative polymerase chain reaction analy-sis.Results The PCL /COLⅠ and PCL /COLⅠ HAP scaffolds had a porous and interwoven structure,while only the PCL /COLⅠ HAP showed a rough surface because of the nHAP particles packaged inside the fibers.The results of the scanning electronic micro-scope and CCK-8 assay showed that periodontal ligament cells adhering to the scaffolds extended adequately and proliferated stably.Ad-ditionally,the real-time quantitative polymerase chain reaction analysis revealed that runt-related transcription factor 2 and osteocalcin of the cells cultured on the PCL /COLⅠ HAP expressed higher than that of the control group,which suggested that the PCL /COLⅠ HAP scaffold had the ability to induce osteogenic differentiation of periodontal ligament cells.Conclusions The PCL /COLⅠ HAP scaffold which has good biocompatibility and osteoinductive ability may serve as a novel biodegradable membrane for guided tissue re-generation.
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