Furan Oxidation Cross-Linking: A New Approach for the Study and Targeting of Peptide/Protein and Nucleic Acid Interactions

摘要

A novel mildly inducible cross-linking methodology for DNA interstrand cross-linking (ICL) was developed by our group, inspired by the metabolic activation of furan moieties (Figure 1). Nucleosides, modified in various ways with furan and easily accessible due to the stability and wide commercial availability of furan derivatives, are incorporated into oligonucleotides (ODNs). After hybridization into a duplex they are oxidized to a very reactive ketobutenal, resulting in selective and high yielding cross-link formation with the opposite base [1]. The strategy has now been investigated for broader application, as the method further holds potential for cross-linking of DNA binding proteins to their target.